Novel properties of bacterial elongation factor Tu.
نویسندگان
چکیده
منابع مشابه
The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants.
Innate immunity is based on the recognition of pathogen-associated molecular patterns (PAMPs). Here, we show that elongation factor Tu (EF-Tu), the most abundant bacterial protein, acts as a PAMP in Arabidopsis thaliana and other Brassicaceae. EF-Tu is highly conserved in all bacteria and is known to be N-acetylated in Escherichia coli. Arabidopsis plants specifically recognize the N terminus o...
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Understanding the molecular mechanism of antibiotics that are currently in use is important for the development of new antimicrobials. The tetracyclines, discovered in the 1940s, are a well-established class of antibiotics that still have a role in treating microbial infections in humans. It is generally accepted that the main target of their action is the ribosome. The estimated affinity for t...
متن کاملStructural investigations of kirromycin bound to bacterial elongation factor Tu by NMR and molecular dynamics.
Kirromycin, also known as mccimycin, is a member of the elfamycin family of antibiotics. These antibiotics inhibit bacterial protein synthesis by binding tightly to elongation factor Tu (Emu) a 43.2 kD G-protein. E m u .GTP is able to carry aminoacyl tRNA to the ribosome in the presence of kirromycin in the usual way, but after hydrolysis of GTP EFTu.GDP is unable to leave the ribosome. Consequ...
متن کاملInhibition of bacterial protein synthesis by elongation-factor-Tu-binding antibiotics MDL 62,879 and efrotomycin.
MDL 62,879 (formerly GE 2270 A) is a novel antibiotic active against Gram-positive bacteria by inhibiting protein synthesis. MDL 62,879 is not active against Gram-negative bacteria, but inhibits cell-free protein synthesis in extracts from Escherichia coli, and shows a high binding affinity for its elongation factor Tu (EF-Tu). We prepared ribosomes and protein-synthesis elongation factors from...
متن کاملAn epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains.
A monoclonal antibody (MAb), MAb 900, which detects a 43-kDa protein present on Escherichia coli was found. Subsequently, more than 90 organisms, belonging to either the bacterial, archaeal, or eucaryal domain, were tested for reactivity to this MAb. Of the bacterial and archaeal domains, almost all species proved to be positive, whereas all organisms from the eucaryal domain gave negative resu...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1978
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.75.3.1250